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By F.J. Dixon, Henry G. Kunkel (Eds.)

ISBN-10: 0120224186

ISBN-13: 9780120224180

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This method is the most sensitive technique presently available to identify the products of individual antibody-producing clones. Every clone which Schlossman observed in more than one animal was seen in both responder and nonresponder guinea pigs. One clone was seen ten times in individual responder guinea pigs and the same clone was observed seven times in nonresponder animals. These results strongly suggest that there is no detectable difference between responder and nonresponder B cell populations.

A ( 4 R ) chromosome resulted from a crossover event between the K region of H - 2 and Ss-SZp genes. Data presented elsewhere indicate that the crossover separates the IT-IgA and IT-ZgG genes occurring in this region, and, thus, Zr-ZgA and Zr-IgG are at sepitratc loci. The 1R kind 2R chroinosonies are conil)ined as the H - 2 products have not yet been distinguished; this is also true of 3 R and 5R. , 197211. , 197213). The results of testing the various recombinants are summarized in Fig.

Were recorded. In order to determine whether this represented a specific inhibitory effect or a nonspecific blocking effect, this experiment was repeated by using lymphocytes from ( 2 X 13)F, guinea pigs. These lymphocytes are capable of responding to both GT and GL and possess the histoconipatibility antigens of both parental strains, 2 and 13. Responses to phytoheniagglutinin (PHA ) were included as a specificity control. Because in all cases the proliferative response to PHA was less inhibited than the response to antigen, the results were expressed as the ratio of response to antigen to the response induced by PHA.

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Advances in Immunology, Vol. 18 by F.J. Dixon, Henry G. Kunkel (Eds.)

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